With chrysanthemum as the test material, 120 stems of chrysanthemum with uniform growth and thick were selected and divided into 2 groups with 60 branches in each group. Cut the leafy branches with a length of about 12cm, and cut the base into a wedge-shaped surface, treat the base with 10PPM naphthalene acid for 12 hours, and then multiply the intelligent seedbed with natural light and the red seedbed with plant growth lights. , Observe and record the growth of the stem. The chlorophyll content is determined by extraction method. On the 3\6\12 days of culture, evenly take 0.2g of the leaves of the same part of each treatment, cut them, soak them with 1:1 acetone:absolute ethanol, and place them in a 40°C incubator After 24 hours of extraction, the OD value at the wavelength of 652nm was measured, and the chlorophyll content was calculated. Soluble sugars were measured by the 3,5-dinitrosalicylic acid method, and the nitrate reductase (NR) activity was measured by the sulfa colorimetric method. The following results were obtained:
1. After 30 days of cultivation, the stems under red light will take root earlier than those under natural light. The final number of roots will be more, and the rooting rate will be as high as 100%, and the roots will be rich and strong. The leaves are dark green, the stems are strong, and the seedlings are growing vigorously. During the whole cultivation process, the growth of the material under red light was obviously better than that under natural light, which showed that red light had the effect of promoting the rooting of the yellow chrysanthemum.
2. During the growth of the stem, whether it is under natural light or red light, the chlorophyll content first decreases and then increases. However, the content of chlorophyll under red light is higher than that under natural light, indicating that red light has a significant promoting effect on the formation of chlorophyll, and this result becomes more obvious with the increase of the number of cultivation days. The better growth of plants under red light may be due to the higher chlorophyll content in the plant, stronger photosynthesis, and more carbohydrate synthesis, which provides sufficient material and energy for plant growth.
3. The soluble sugar content on the 9th day of culture was lower than that on the 15th day, and it decreased more under red light than under natural light. The stems under red light also took root earlier than under natural light. After 15 days, the soluble sugar content under red light is higher than that under natural light, which may be related to the higher chlorophyll content under red light and stronger photosynthesis.
4. The activity of NR in the stem under red light was significantly greater than that under natural light. It can be seen that red light can promote nitrogen metabolism in the stem of chrysanthemum.
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